Cover of: Freeze-fracture replication of biological tissues | C. Stolinski

Freeze-fracture replication of biological tissues

techniques, interpretation, and applications
  • 173 Pages
  • 3.13 MB
  • English
Academic Press , London, New York
Freeze fractu
StatementC. Stolinski, A. S. Breathnach.
ContributionsBreathnach, A. S., joint author.
LC ClassificationsQH236.2 .S76
The Physical Object
Paginationviii, 173 p. :
ID Numbers
Open LibraryOL5197304M
ISBN 100126720509
LC Control Number75019678

Get this from a library. Freeze-fracture replication of biological tissues: techniques, interpretation, and applications. [C Stolinski; A S Breathnach]. Freeze-fracture Replication of Biological Tissues: Techniques, Interpretation and Applications by C.

Stolinski, A.S. Breathnach and a great selection of related books, art and collectibles available now at Freeze-fracture replication technique of human skin. Caputo R, Gasparini G. The freeze-fracture replication technique represents one of the most advanced methods for studying the ultrastructure of biological tissues and has given a new dimension to the ultrastructure by: 8.

Description Freeze-fracture replication of biological tissues FB2

Introduction. The concept and practical application of freeze-fracture processing of biological specimens was introduced by Steere 1 over half a century ago. The early apparatus appropriated disparate components into a working self-contained unit original apparatus was modified and refined into commercially available instruments in order to accommodate the critical need Cited by: 3.

Freeze fracture is the only way to determine the presence of such junctions. The method is also good for the study of intramembrane structures.

Sample Preparation for Freeze Fracture. Fix sample (typically glutaraldehyde) Cryoprotect Glycerol, Sucrose, Ethylene glycol, DMSO; Freeze sample Cryogens Propane (°C) Liquid nitrogen (°C).

Book Reviews Book Reviews Book reviewed in this article: Cytodifferentiation in Plants—Xylogenesis as a Model System. By L.

Details Freeze-fracture replication of biological tissues PDF

Roberts Freeze‐fracture Replication of Biological Tissues. Techniques, Interpretation and Applications. By C. Stolinski and A.

Breathnach Advances in Optical and Electron Microscopy. Download PDF: Sorry, we are unable to provide the full text but you may find it at the following location(s): g (external link)Author: A.

Boyde. Micron,Vol. Pergamon Press. Printed in Great Britain. Freeze-fracture repHcatlon in biological research: Development, current practice and future prospects C. STOLINSKI Department of Anatomy, St. ~Iary's Hospital Medical School, London 1PG, England, U.K.

Invited Review: Received Ap INTRODUCTION In recent years, Cited by: The concept and practical application of freeze-fracture processing of biological specimens was introduced by Steere 1 over half a century ago.

The early apparatus appropriated disparate components into a working self-contained unit original apparatus was modified and refined into commercially available instruments in order to accommodate the critical need for remote Cited by: 3. Stolinski, C. () Freeze-fracture replication in biological research -- Development, current practice and future prospects, Micron, 8: Stolinski, C.

and A. Breathnach () Freeze-Fracture Replication of Biological Tissues -- Techniques, Interpretation and Applications, Academic Press, by:   Freeze fracture depends on the property Freeze-fracture replication of biological tissues book frozen tissues or cells, when cracked open, to split along the hydrophobic interior of membranes, thus revealing broad panoramas of membrane interior.

These Author: Douglas E. Chandler, William P. Sharp. freeze-fracturing: [ frēz´-frak″chur-ing ] a method of preparing cells for electron-microscopical examination: a tissue specimen is frozen at −°C, inserted into a vacuum chamber, and fractured by a microtome; a platinum carbon replica of the exposed surfaces is made, freed of the underlying specimen, and then examined.

While the book emphasizes freeze fracture images and the useful scientific information contained in them, the authors have also included transmission electron micrographs of ultrathin sectioned cells, tissues, and organs in order to aid in the interpretation of the freeze fracture image and increase the book's utility.

Where three dimensional Cited by: 3. Freeze fracture definition is - preparation of a specimen (as of biological tissue) for electron microscopic examination by rapid freezing, fracturing along natural structural lines, and preparing a replica of the exposed structural details (as by simultaneous vapor deposition of carbon and platinum).

How to use freeze fracture in a sentence. FFRT - Freeze-Fracture Replication Technique. Looking for abbreviations of FFRT. It is Freeze-Fracture Replication Technique.

Freeze-Fracture Replication Technique listed as FFRT. Freeze-Fracture Replication Technique - How is Freeze-Fracture Replication Technique abbreviated. Freeze-Fracture Replication Technique; Freeze-Fracture Thaw-Fix. Freeze Fracture and Freeze Etching I. PRINCIPLES OF FREEZE FRACTURE TECHNIQUE The technique of freeze fracture is unique among electron microscopic (EM) methods in that it gives en face views of the internal organisation of biological membranes, allowing the study of the in-plane distribution of integral proteins spanning the lipid bilayer and of other membrane.

Freeze-fracture techniques are based on the mechanical fracture of frozen biological material and the high-resolution metal foil replication of the exposed surfaces. Replication by vapor deposition of platinum and carbon reveals transmembrane proteins as intramembrane particles (IMPs) at ca.

1 nm topgraphic resolution. Freeze-fracture electron microscopy (FEM) is a unique and powerful research technique that provides a triad of major advantages for the microbiologist interested in Cited by: 9.

An electron microscope is a microscope that uses a beam of accelerated electrons as a source of illumination. As the wavelength of an electron can be up totimes shorter than that of visible light photons, electron microscopes have a higher resolving power than light microscopes and can reveal the structure of smaller objects.

A scanning transmission electron microscope. Freeze fracture describes the technique of breaking a frozen specimen to reveal internal structures. Freeze etching is the sublimation of surface ice under vacuum to reveal details of the fractured face that were originally hidden. A metal/carbon mix enables the sample to be imaged in a SEM (block-face) or TEM (replica).

It is used to investigate for instance cell organelles. Biological Sciences Freeze Fracture Studies Of Membranes 1st Edition. by Sek Wen Hui (Author) ISBN ISBN Why is ISBN important. ISBN. This bar-code number lets you verify that you're getting exactly the right version or edition of a book.

Cited by: The sample was then prepared for electron microscopy by freeze-fracture replication which produces a platinum shadowed replica of the fractured tissue surface, with white shadows. Membranes can fracture down the centre of the bilayer producing an external (E) face and a protoplasmic (P) face.

freeze-fracture technique: Definition: Search for: Biology Glossary search by A technique used to look at membranes that reveal the pattern of integral membrane proteins. General outline of technique: 1. Cells are quickly frozen in liquid nitrogen (C), which immobilizes cell components instantly.

fully guides the reader through mounting tissues and cut- ting, mounting, and staining sections. Instrumentation also is examined and discussed. Freeze-drying and freeze-fracture replication are in- cluded in Chapters 5 and 6. Neither of these general topics is new to microscopists; freeze-drying predates critical-pointAuthor: David C.

Joy. The frozen-hydrated specimen is the principal element that unifies the subject of low- temperature microscopy, and frozen-hydrated specimens are what this book is all about.

Rating: (not yet rated) 0 with reviews - Be the first. Drug delivery systems are defined as formulations aiming for transportation of a drug to the desired area of action within the body. The basic component of drug delivery systems is an appropriate carrier that protects the drug from rapid degradation or clearance and thereby enhances drug concentration in target tissues.

Based on their biodegradable, biocompatible, Cited by: Freeze-fracture electron microscopy of shrunken cells identified major alterations in the organization of the cell envelope. Invaginations typical of the yeast plasma membrane (Fig.

10a). A book entitled Food Microscopy by Olga Flint and replication of either dehydrated or frozen and freeze-fractured samples is yet another possibility.

Each of these procedures has some advantages and some drawbacks. Freeze-Fracture Replication of Biological Tissues C. Stolinski and A. Breathnach, pp., Academic Press, 5. Electron Tomography of Biological Macromolecules (W Baumeister et al.) Leydig Cell — A Normal Cell Model of Cellular Autophagy (X M Tang et al.) Ultrastructural Analysis of Regenerating Explants (K Pihakaski-Maunsbach et al.) Ultrastructural Study of Spermatogenic Cells of the Gastropod Pila Luzonica (B C Ramos).

Gap junctions are a specialized intercellular connection between a multitude of animal cell-types. They directly connect the cytoplasm of two cells, which allows various molecules, ions and electrical impulses to directly pass through a regulated gate between cells.

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One gap junction channel is composed of two connexons (or hemichannels), which connect across the FMA:. Procedure for the formation of freeze-fracture replicas as described in the text: Freeze etching is an optional step in which a thin layer of covering ice is evaporated to reveal additional information about the structure of the fractured specimen.-In freeze-fracture replication, frozen tissue is fractured with a knife.3.

FUNDAMENTAL PROCESSES. A) DNA replication, repair, and recombination: Unit of replication, enzymes involved, replication origin and replication fork, the fidelity of replication, extrachromosomal replicons, DNA damage and repair mechanisms, homologous and site-specific recombination.

B) RNA synthesis and processing: Transcription factors and machinery, a. Freeze-Fracture Replication of Pyramidal Cells Application Note for Leica EM HPM - Frozen samples (90 μm thick slices frozen by HPM) were inserted into a double replica table and then fractured into two pieces at –°C (after insertion of.